Journal: International Journal of Molecular Sciences
Article Title: Methodological Comparison of Short-Read and Long-Read Sequencing Methods on Colorectal Cancer Samples
doi: 10.3390/ijms26189254
Figure Lengend Snippet: Variant allele frequency (VAF) values from Illumina exome panel restrictions using Nanopore and Illumina platforms. ( a ) displays results for the NEG group. ( b ) displays results for the NAT group, and ( c ) for CRC morphological classifications. The x-axis represents gene–mutation pairs, while the y-axis shows their corresponding VAF values. Blue circles indicate data from Nanopore sequencing, and red circles represent Illumina-derived values. Arrows indicate the direction of VAF differences: upward when Illumina VAF is greater than Nanopore, and downward when it is lower.
Article Snippet: Therefore, no statistically significant differences in mean VAF values can be established between the two platforms for mutations commonly detected within the Illumina exome panel target regions.
Techniques: Variant Assay, Mutagenesis, Nanopore Sequencing, Derivative Assay